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G418 DISULFATE CELL CULTURE TESTED

Geneticin

CAS: 108321-42-2

Molecular Formula: C20H42N4O14S

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G418 DISULFATE CELL CULTURE TESTED - Names and Identifiers

Name Geneticin
Synonyms Geneticin
G418 Sulfate
G418 SOLUTION
G-418 Sulfate
sulfuric acid
G-418 SULPHATE
ANTIBIOTIC G-418 Sulfate
G-418 disulfate,Geneticin
G418 DISULFATE CELL CULTURE TESTED
TRYPSIN INHIBITOR SOYBEAN REAGENT GRADE
G418 DISULFATE PLANT CELL CULTURE TESTED
Geneticin Disulfate (G418), Antibiotic for Culture Media Use Only
G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested
2-[4,6-diamino-3-[3-amino-4,5-dihydroxy-6-(1-hydroxyethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxy-5-methyl-4-(methylamino)oxane-3,5-diol,sulfuric acid
(2R,3S,4R,5R,6S)-5-amino-6-[(1S,2R,4S,6R)-4,6-diamino-3-[(3R,4R,5R)-3,5-dihydroxy-5-methyl-4-methylamino-tetrahydropyran-2-yl]oxy-2-hydroxy-cyclohexoxy]-2-(1-hydroxyethyl)tetrahydropyran-3,4-diol
CAS 108321-42-2
EINECS 600-864-4
InChI InChI=1/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9-,10-,11+,12-,13-,14-,15+,16?,17-,18-,19?,20+;;/m1../s1
InChIKey QNQZPJLBGRQFDD-ZLRXUJDMSA-N

G418 DISULFATE CELL CULTURE TESTED - Physico-chemical Properties

Molecular FormulaC20H42N4O14S
Molar Mass594.63
Boling Point1012.1°C at 760 mmHg
Flash Point565.9°C
Water Solubilitysoluble
Solubility Soluble in water and methanol.
Vapor Presure0mmHg at 25°C
AppearanceWhite to white-like
Colorwhite to off-white
Storage Condition2-8°C
MDLMFCD00058314
Physical and Chemical PropertiesSolubility: H2O: 50 mg/mL
storage condition: 2-8 ℃
WGK Germany:3
In vitro studyG418 is an inhibitor of many prokaryotic and eukaryotic organisms at concentrations of 1-300 μg/ml. Resistance to G418 by the neo gene of Tn5 encoding aminoglycoside 3 '-phosphotransferase, APT 3'II is commonly used in laboratory studies to select genetically engineered cells [1]. Typically, for bacteria and algae a concentration of 5mg / L or less is used, for mammalian cells a concentration of about 400mg / L is used for selection and 200mg / L is used for maintenance. Selection of resistant clones may take 1 to 3 weeks [2].
In vivo studyThree consecutive days of G418(40 and 80 mg/kg) were sufficient to eliminate all untransfected Brucella herpesvirus parasites in infected mice.

G418 DISULFATE CELL CULTURE TESTED - Risk and Safety

Risk CodesR20/21/22 - Harmful by inhalation, in contact with skin and if swallowed.
R36/37/38 - Irritating to eyes, respiratory system and skin.
R42/43 - May cause sensitization by inhalation and skin contact.
Safety DescriptionS22 - Do not breathe dust.
S24/25 - Avoid contact with skin and eyes.
S36/37 - Wear suitable protective clothing and gloves.
S23 - Do not breathe vapour.
S36 - Wear suitable protective clothing.
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
WGK Germany3
RTECSCB9378500
HS Code29419090

G418 DISULFATE CELL CULTURE TESTED - Reference

Reference
Show more
1. Zhang Jian, Zhao Suping, Lu Samei, et al. Expression of ZBTB7A in Hepatocellular Carcinoma and Its Correlation with Tumor Drug Resistance [J]. Journal of Clinical and Experimental Pathology, 2019(10).
2. Zhou Qinqin. Metabolic Engineering Modification and Fermentation Process Optimization of Lycopene Synthesis by Saccharomyces cerevisiae [D]. Jiangnan University, 2020.
3. [IF = 5.037] Min Ji et al."The cooperative complex of Argonaute-2 and microRNA-146a regulates hepatitis B virus replication through flap endonuclease 1." Life Sci. 2020 Sep;257:118089
4. [IF = 2.926] Wang Deqing et al."Production of a Recombinant α-l-Rhamnosidase from Aspergillus niger CCTCC M 2018240 in Pichia pastoris." Appl Biochem Biotech. 2019 Nov;189(3):1020-1037

G418 DISULFATE CELL CULTURE TESTED - Introduction

G418 is an aminoglycoside antibiotic. The structure of this aminoglycoside antibiotic is similar to neomycin, gentamicin, and kanamycin. In molecular genetic tests, it is the most commonly used resistance screening reagent for stable transfection. It blocks protein synthesis by inhibiting the genes of transposon Tn601,Tn5, interfering with ribosome function, and produces toxins to prokaryotic and eukaryotic cells, including bacteria, yeast, plant and mammalian cells, as well as protozoa and worms. When the neo gene is integrated into the eukaryotic cell DNA, it can initiate the transcription of the sequence encoded by the neo gene into mRNA, thereby obtaining the high-efficiency expression of the resistance product aminoglycoside phosphotransferase, so that the cell can obtain resistance and be able to contain G418 Growth in selective medium blocks polypeptide synthesis by interfering with ribosome function. Not a commonly used standard antibiotic, it is often used to select prokaryotic and eukaryotic cells transfected with neomycin resistance gene. It is widely used in gene transfer, gene knockout, resistance screening and transgenic animals.
Last Update：2022-10-16 17:11:57

G418 DISULFATE CELL CULTURE TESTED - Reference Information

Animal experiment In order to characterize the sensitivity of the trypanosome population to G418 in vivo, the blood flow forms of Brucella brucei GUTat 3.1 and Brucella brucei GUTat 3.1 / BBR3 were amplified separately in mice with sublethal radiation. Before the first peak of parasitemia, trypanosomes were collected and aliquots containing 106 trypanosomes were intraperitoneally inoculated into mice. 24 hours after infection, the mice were divided into groups and treated with G418 at doses of 10,20,30,40,50 or 80mg / kg body weight (bw) by intraperitoneal inoculation of 0.2mL of the drug. In sterile water. At 24 and 48 hours after the first treatment, G418 was administered to each group of animals at the same dose as before, resulting in three treatments per mouse. Repeated drug therapy is required to ensure complete elimination of untransfected GUTat 3.1 parasites from mice. The mice were then monitored daily for 33 days by microscopic examination of the wet blood membrane to detect the presence of parasites. The animals where the parasite was found were recorded and then removed from the experiment.
biological activity Geneticin (G418 Sulfate), an aminoglycoside antibiotic, is an inhibitor of 80 S ribosomes extension, in both prokaryotic and eukaryotic cells It can block polypeptide synthesis by inhibiting the extension step.
use blocks polypeptide synthesis by interfering with ribosome function. Not a commonly used standard antibiotic, it is often used to select prokaryotic and eukaryotic cells transfected with neomycin resistance gene. It is widely used in gene transfer, gene knockout, resistance screening and transgenic animals.
Last Update：2024-04-09 21:04:16
G418 DISULFATE CELL CULTURE TESTED
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Tel: 18301782025
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Mobile: 18021002903
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Hubei YoungXin Pharmaceutical Tech Co.,Ltd.
Product Name: Geneticin  Disulfate Salt Request for quotation
CAS: 108321-42-2
Tel: 0714-3999186
Email: 2853786052@qq.com
Mobile: 86+15671228036
QQ: 2853786052 Click to send a QQ message
Wechat: 15671228036
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Product Name: Geneticin Request for quotation
CAS: 108321-42-2
Tel: +86 19849939632
Email: 2470479589@qq.com
Mobile: +86 19849939632
QQ: 2470479589 Click to send a QQ message
Wechat: +86 19849939632
Hefei TNJ Chemical Industry Co.,Ltd.
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CAS: 108321-42-2
Tel: 0086-551-65418684
Email: sales@tnjchem.com
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